sch529074 compound (MedChemExpress)
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sch529074 compound
Sch529074 Compound, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sch529074+compound/SCH529074/pmc12052144-377-9-11
Average 93 stars, based on 3 article reviews
Sch529074 Compound, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sch529074+compound/SCH529074/pmc12052144-377-9-11
Average 93 stars, based on 3 article reviews
sch529074 compound - by Bioz Stars,
2026-09
93/100 stars
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1) Product Images from "iSoMAs: Finding isoform expression and somatic mutation associations in human cancers"
Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers
Journal: PLOS Computational Biology
doi: 10.1371/journal.pcbi.1012847
Figure Legend Snippet: (A) Western blot generated from NCI-H1975 cell lysates. Cells were treated with 6µM SCH529074 compound (+) or mock DMSO (-) for 24 hours. Expression of p53 and p21 is shown. Vinculin was included as a loading control. (B) Differential isoform expression for CDKN1A (encoding p21) upon TP53 mutation in both clinical (TCGA-LUAD) and cell line (NCI-H1975) samples as indicated. (C) Differential isoform expression for TPX2 and NCAPG upon TP53 mutation in both clinical (TCGA-LUAD) and cell line (NCI-H1975) samples as indicated. (D) Signaling pathway enrichment analysis on the top 100 isoform targets (87 unique genes) of TP53 in LUAD against 343 KEGG pathways. Only pathways with Hypergeometric test P<0.05 are shown. (E) Survival analysis based on the mutation status of TP53 in LUAD cancer. (F) Survival analysis based on the expression level of two representative isoform targets of TP53 in LUAD as indicated. LUAD tumor samples were divided into 3 tiers equally based on expression level of each isoform, and only the high (n=171) and low (n=171) tiers were kept for comparison. (G) Differential expression analysis for two representative isoform targets of TP53 between normal (n=59) and tumor (n=517) LUAD samples. (H) A representative result of the cell cycle analysis for NCI-H1975 cells under various treatment conditions at different time points as indicated. (I) Cell apoptosis analysis for NCI-H1975 cells under various treatment conditions at different time points as indicated. Percent of cells are presented as MeanSD for 2 replicates. P-values for survival analysis were derived from log-rank test; Significance levels for differential analysis were derived from Wilcoxon rank-sum test (for TCGA data) or t -test (for H1975 cell line data), **** P <1e-4, *** P <1e-3, ** P <0.01, * P <0.05, ns: non-significant. See also and .
Techniques Used: Western Blot, Generated, Expressing, Control, Mutagenesis, Comparison, Quantitative Proteomics, Cell Cycle Assay, Derivative Assay
Related Articles
Western Blot:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Generated:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Expressing:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Control:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Mutagenesis:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Comparison:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Quantitative Proteomics:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Cell Cycle Assay:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Derivative Assay:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Nucleic Acid Electrophoresis:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Quantitative RT-PCR:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Standard Deviation:Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers Article Snippet: Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.Cells were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight.. Culture medium was replaced with medium containing Article Title: iSoMAs: Finding isoform expression and somatic mutation associations in human cancers. Article Snippet: cells PLOS Computational Biology | https://doi.org/10.1371/journal.pcbi.1012847 March 7, 2025 29 / 39 per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM Article Title: SoMAS: Finding somatic mutations associated with alternative splicing in human cancers Article Snippet: were initially seeded at 0.5 x 10 5 cells per well in a 6 well plate and grown overnight. .. Culture medium was replaced with medium containing 6 µM |